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Calnexin Antibody (IE2.1C12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36571

Novus Biologicals, part of Bio-Techne
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NBP2-36571SS
NBP2-36571

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (95%), Mouse (94%), Primate (99%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # IE2.1C12

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Partial recombinant human Calnexin protein (between amino acids 1-300) [UniProt# P27824]

Localization

Endoplasmic reticulum membrane, Melanosome

Marker

Endoplasmic Reticulum Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Calnexin Antibody (IE2.1C12) - BSA Free

Western Blot: Calnexin Antibody (IE2.1C12)BSA Free [NBP2-36571]

Western Blot: Calnexin Antibody (IE2.1C12)BSA Free [NBP2-36571]

Western Blot: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone IE2.1C12) at 0.5 ug/ml concentration.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (IE2.1C12) - BSA Free [NBP2-36571]

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (IE2.1C12) - BSA Free [NBP2-36571]

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Calnexin (1E2.1C12) antibody was tested in Hela cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - BSA Free [NBP2-36571]

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - BSA Free [NBP2-36571]

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cells of Glomeruli developed strong cytoplasmic staining.

Applications for Calnexin Antibody (IE2.1C12) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

5 ug/million cells

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

7 ug/ml

Immunohistochemistry-Paraffin

7 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, p90

Entrez Gene IDs

821 (Human); 12330 (Mouse); 29144 (Rat)

Gene Symbol

CANX

UniProt

Additional Calnexin Products

Product Documents for Calnexin Antibody (IE2.1C12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Calnexin Antibody (IE2.1C12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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