Calnexin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-37774

Novus Biologicals, part of Bio-Techne
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NBP1-37774
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Opossum

Predicted:

Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Calnexin Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SKTPELNLDQFHDKT corresponding to internal region (near N-Terminus) according to NP_001737.1.

Specificity

Reported variants represent identical protein (NP_001019820.1, NP_001737.1).

Marker

Endoplasmic Reticulum Membrane Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Calnexin Antibody

Western Blot: Calnexin Antibody [NBP1-37774]

Western Blot: Calnexin Antibody [NBP1-37774]

Western Blot: Calnexin Antibody [NBP1-37774] - Staining of CaCo-2 (A) with antibody at 0.1 ug/mL and NIH3T3 (B) cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NBP1-37774]

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NBP1-37774]

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NBP1-37774] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-37774]

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-37774]

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-37774] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for Calnexin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 1 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin, an abundant approx. 90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their Cterminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, p90

Entrez Gene IDs

821 (Human); 12330 (Mouse); 29144 (Rat)

Gene Symbol

CANX

UniProt

P27824 (Human)

Additional Calnexin Products

Product Documents for Calnexin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calnexin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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