Calnexin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97485

Novus Biologicals, part of Bio-Techne
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NBP1-97485-0.025mg
NBP1-97485-0.05mg
NBP1-97485-0.2mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Primate, Rabbit, Sheep, Xenopus

Cited:

Human, Mouse, Rat, Porcine, Canine, Primate

Applications

Validated:

Electron Microscopy, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calnexin Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the sequence near the C-terminus of canine calnexin.

Reactivity Notes

Quail (100%).

Marker

Endoplasmic Reticulum Membrane Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calnexin Antibody

Western Blot: Calnexin Antibody [NBP1-97485]

Western Blot: Calnexin Antibody [NBP1-97485]

Western Blot: Calnexin Antibody [NBP1-97485] - Analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).
Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97485]

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97485]

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97485] - Analysis of human spleen tissue stained with Calnexin, pAb at 10ug/ml.

Applications for Calnexin Antibody

Application
Recommended Usage

Electron Microscopy

1:10-1:500

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Simple Western

1:250

Western Blot

1:1000
Application Notes
Detects a band of ~90kDa by Western blot. Flow Cytometry was reported in scientific literature. Simple Western reported by an internal validation: tested in human multiple myeloma; antibody dilution of 1:250; separated by size; detects a band at 119 kDa
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins.

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, p90

Entrez Gene IDs

821 (Human); 12330 (Mouse); 29144 (Rat); 403908 (Canine)

Gene Symbol

CANX

UniProt

P24643 (Canine)

Additional Calnexin Products

Product Documents for Calnexin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calnexin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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