Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7D2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Calpain 5 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 274-578 of human CAPN5 (NP_004046) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpain 5 Antibody (OTI7D2) - Azide and BSA Free

Immunohistochemistry: Calpain 5 Antibody (OTI7D2) - Azide and BSA Free [NBP2-70328]

Immunohistochemistry: Calpain 5 Antibody (OTI7D2) - Azide and BSA Free [NBP2-70328]

Immunohistochemistry: Calpain 5 Antibody (OTI7D2) - Azide and BSA Free [NBP2-70328] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: Calpain 5 Antibody (OTI7D2) - Azide and BSA Free [NBP2-70328]

Immunohistochemistry: Calpain 5 Antibody (OTI7D2) - Azide and BSA Free [NBP2-70328]

Immunohistochemistry: Calpain 5 Antibody (OTI7D2) - Azide and BSA Free [NBP2-70328] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Applications for Calpain 5 Antibody (OTI7D2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calpain 5

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq]

Alternate Names

calpain 5, Calpain htra-3, calpain-5, EC 3.4.22, EC 3.4.22.54, FLJ46245, HTRA3NCL3, nCL-3EC 3.4.22.-, New calpain 3

Gene Symbol

CAPN5

Additional Calpain 5 Products

Product Documents for Calpain 5 Antibody (OTI7D2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Calpain 5 Antibody (OTI7D2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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