Calpain 5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32222
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (95%), Rat (92%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Calpain 5 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 5. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (81%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calpain 5 Antibody
![Immunocytochemistry/ Immunofluorescence: Calpain 5 Antibody [NBP1-32222]](https://resources.bio-techne.com/images/products/Calpain-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32222-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Calpain 5 Antibody [NBP1-32222]")
Immunocytochemistry/ Immunofluorescence: Calpain 5 Antibody [NBP1-32222]
Immunocytochemistry/Immunofluorescence: Calpain 5 Antibody [NBP1-32222] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.
Western Blot: Calpain 5 Antibody [NBP1-32222] -
Western Blot: Calpain 5 Antibody [NBP1-32222] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (NBP1-32222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Calpain 5 Antibody [NBP1-32222] -
Western Blot: Calpain 5 Antibody [NBP1-32222] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (NBP1-32222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Calpain 5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Calpain 5
Alternate Names
calpain 5, Calpain htra-3, calpain-5, EC 3.4.22, EC 3.4.22.54, FLJ46245, HTRA3NCL3, nCL-3EC 3.4.22.-, New calpain 3
Gene Symbol
CAPN5
Additional Calpain 5 Products
Product Documents for Calpain 5 Antibody
Product Specific Notices for Calpain 5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.