Calpain S1 Antibody (1D11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59446

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D11

Format

BSA Free

Concentration

1 mg/ml

View all Calpain S1 Antibodies »

Product Specifications

Immunogen

Recombinant human Calpain S1 (84-268aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Calpain S1 Antibody (1D11) - BSA Free

Western Blot: Calpain S1 Antibody (1D11)BSA Free [NBP2-59446]

Western Blot: Calpain S1 Antibody (1D11)BSA Free [NBP2-59446]

Western Blot: Calpain S1 Antibody (1D11) [NBP2-59446] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calpain S1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A431 cell lysate Lane 3.: 293T cell lysate
Immunocytochemistry/ Immunofluorescence: Calpain S1 Antibody (1D11) - BSA Free [NBP2-59446]

Immunocytochemistry/ Immunofluorescence: Calpain S1 Antibody (1D11) - BSA Free [NBP2-59446]

Immunocytochemistry/Immunofluorescence: Calpain S1 Antibody (1D11) [NBP2-59446] - Analysis of Calpain S1 in HeLa cells. The cell was stained with (1:100). The secondary antibody (green) was used Alexa Fluor 488.

Applications for Calpain S1 Antibody (1D11) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calpain S1

This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Alternate Names

Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit 1, calcium-dependent protease, small subunit, calpain 4, small subunit (30K), Calpain regulatory subunit, calpain small subunit 1, calpain, small polypeptide, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1, epididymis secretory sperm binding protein

Gene Symbol

CAPNS1

Additional Calpain S1 Products

Product Documents for Calpain S1 Antibody (1D11) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calpain S1 Antibody (1D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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