Calpain S1 Antibody (3C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00000826-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calpain S1 Antibodies »

Product Specifications

Immunogen

Calpain S1 (AAH00592, 172 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLYNMIIRRYSDESGNMDFDNFISCLVRLDAMFRAFKSLDKDGTGQIQVNIQE

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Calpain S1 Antibody (3C4)

Western Blot: Calpain S1 Antibody (3C4) [H00000826-M01]

Western Blot: Calpain S1 Antibody (3C4) [H00000826-M01]

Western Blot: Calpain S1 Antibody (3C4) [H00000826-M01] - Analysis of Calpain S1 expression in transfected 293T cell line by Calpain S1 monoclonal antibody (M01), clone 3C4.Lane 1: Calpain S1 transfected lysate(28.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Calpain S1 Antibody (3C4) [H00000826-M01]

Immunocytochemistry/ Immunofluorescence: Calpain S1 Antibody (3C4) [H00000826-M01]

Immunocytochemistry/Immunofluorescence: Calpain S1 Antibody (3C4) [H00000826-M01] - Analysis of monoclonal antibody to Calpain S1 on HeLa cell. Antibody concentration 20 ug/ml.
Immunohistochemistry-Paraffin: Calpain S1 Antibody (3C4) [H00000826-M01]

Immunohistochemistry-Paraffin: Calpain S1 Antibody (3C4) [H00000826-M01]

Immunohistochemistry-Paraffin: Calpain S1 Antibody (3C4) [H00000826-M01] - Analysis of monoclonal antibody to Calpain S1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
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Applications for Calpain S1 Antibody (3C4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calpain S1

This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Alternate Names

Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit 1, calcium-dependent protease, small subunit, calpain 4, small subunit (30K), Calpain regulatory subunit, calpain small subunit 1, calpain, small polypeptide, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1, epididymis secretory sperm binding protein

Entrez Gene IDs

826 (Human)

Gene Symbol

CAPNS1

OMIM

114170 (Human)

UniProt

AAH00592 (Human)

Additional Calpain S1 Products

Product Documents for Calpain S1 Antibody (3C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpain S1 Antibody (3C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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