Calpain S1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06243

Novus Biologicals, part of Bio-Techne
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NBP3-06243-100ul
NBP3-06243-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calpain S1 Antibodies »

Product Summary for Calpain S1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human Calpain S1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Calpain S1 Antibody

Western Blot: Calpain S1 Antibody [NBP3-06243]

Western Blot: Calpain S1 Antibody [NBP3-06243]

Western Blot: Calpain S1 Antibody [NBP3-06243] - Anti-Calpain S1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: K562 Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:28 kDa
Immunocytochemistry/ Immunofluorescence: Calpain S1 Antibody [NBP3-06243]

Immunocytochemistry/ Immunofluorescence: Calpain S1 Antibody [NBP3-06243]

Immunocytochemistry/Immunofluorescence: Calpain S1 Antibody [NBP3-06243] - Immunofluorescence staining of Calpain S1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human Calpain S1 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.
Immunohistochemistry-Paraffin: Calpain S1 Antibody [NBP3-06243]

Immunohistochemistry-Paraffin: Calpain S1 Antibody [NBP3-06243]

Immunohistochemistry-Paraffin: Calpain S1 Antibody [NBP3-06243] - Immunochemical staining of human Calpain S1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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Applications for Calpain S1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calpain S1

This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Alternate Names

Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit 1, calcium-dependent protease, small subunit, calpain 4, small subunit (30K), Calpain regulatory subunit, calpain small subunit 1, calpain, small polypeptide, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1, epididymis secretory sperm binding protein

Gene Symbol

CAPNS1

Additional Calpain S1 Products

Product Documents for Calpain S1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Calpain S1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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