Calponin 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36741
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DPGEVPEYPPYYQEE corresponding to internal region (near C-Terminus) according to NP_004359.1, NP_958434.1.
Epitope
internal region (near C Terminus)
Specificity
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with CCN3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Calponin 2 Antibody
![Western Blot: Calponin 2 Antibody [NBP1-36741]](https://resources.bio-techne.com/images/products/Calponin-2-Antibody-Western-Blot-NBP1-36741-img0002.jpg "Western Blot: Calponin 2 Antibody [NBP1-36741]")
Western Blot: Calponin 2 Antibody [NBP1-36741]
Western Blot: Calponin 2 Antibody [NBP1-36741] - Analysis of Calponin 2 in HepG2 lysates (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using this antibody at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Calponin 2 Antibody [NBP1-36741]](https://resources.bio-techne.com/images/products/Calponin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-36741-img0003.jpg "Immunohistochemistry-Paraffin: Calponin 2 Antibody [NBP1-36741]")
Immunohistochemistry-Paraffin: Calponin 2 Antibody [NBP1-36741]
Immunohistochemistry-Paraffin: Calponin 2 Antibody [NBP1-36741] - (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Calponin 2 Antibody
Application
Recommended Usage
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in HepG2 lysates (calculated MW of 33.7 kDa band according to NP_004359.1).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Placenta.
IHC: Paraffin embedded Human Placenta.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Calponin 2
Alternate Names
calponin 2, Calponin H2, smooth muscle, calponin-2, Neutral calponin
Entrez Gene IDs
1265 (Human)
Gene Symbol
CNN2
UniProt
Additional Calponin 2 Products
Product Documents for Calponin 2 Antibody
Product Specific Notices for Calponin 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...