Calponin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25343

Novus Biologicals, part of Bio-Techne
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NBP3-25343
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calponin 2 Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein Calponin 2 using the following amino acid sequence: RRHIYDTKLGTDKCDNSSMSL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calponin 2 Antibody

Calponin 2 Antibody Immunocytochemistry/Immunofluorescence: Calponin 2 Antibody [NBP3-25343]

Immunocytochemistry/Immunofluorescence: Calponin 2 Antibody [NBP3-25343]

Staining of human cell line RT-4 shows localization to nucleoplasm & actin filaments.
Calponin 2 Antibody Western Blot: Calponin 2 Antibody [NBP3-25343]

Western Blot: Calponin 2 Antibody [NBP3-25343]

Analysis in human cell line RT-4 and human cell line U-251 MG.

Applications for Calponin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml

Western Blot

0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calponin 2

Calponin, a 34 kDa protein, regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an actin- and tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-actin and inhibition of the actin-activated MgATPase activity of phosphorylated myosin.Both properties are lost following phosphorylation (primarily at serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin), and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.

Alternate Names

calponin 2, Calponin H2, smooth muscle, calponin-2, Neutral calponin

Gene Symbol

CNN2

Additional Calponin 2 Products

Product Documents for Calponin 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calponin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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