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Calreticulin-2/CALR3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33390

Novus Biologicals, part of Bio-Techne
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NBP2-33390
NBP2-33390-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FLITDDEEYADNFGKATWGETKGPEREMDAIQAKEEMKKAREEEEEELLSGKINRHEHYFNQFHRRN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calreticulin-2/CALR3 Antibody

Immunohistochemistry: Calreticulin-2/CALR3 Antibody [NBP2-33390]

Immunohistochemistry: Calreticulin-2/CALR3 Antibody [NBP2-33390]

Immunohistochemistry: Calreticulin-2/CALR3 Antibody [NBP2-33390] - Examples of immunohistochemical staining for Calreticulin-2/CALR3 (antibody NBP2-33390) in normal and affected tissues. No expression in myocardial tissue from (a) patient with c.564del-positive DCM, b patient with ischemic cardiomyopathy, and c child, d newborn, and e fetus with structurally normal hearts. Instead, we observed positive staining of arteriolar smooth muscle cells in all samples (arrows). f High expression of CALR3 in testicular germ cells as positive control tissue. Scale bars: 1 mm Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41431-018-0208-1), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390] - Staining of human testis shows strong cytoplasmic positivity in spermatids.
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390] - Staining of human endometrium shows low expression as expected.

Applications for Calreticulin-2/CALR3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calreticulin-2

Calreticulins, such as CALR3, are Ca(2+)-binding chaperones localized mainly in the endoplasmic/sarcoplasmic reticulum Persson et al. (2002) [PubMed 12384296].[supplied by OMIM]

Alternate Names

CALR3, Calreticulin2, Crt2

Gene Symbol

CALR3

UniProt

Additional Calreticulin-2 Products

Product Documents for Calreticulin-2/CALR3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Calreticulin-2/CALR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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