Calreticulin-2/CALR3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33390
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FLITDDEEYADNFGKATWGETKGPEREMDAIQAKEEMKKAREEEEEELLSGKINRHEHYFNQFHRRN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Calreticulin-2/CALR3 Antibody
Immunohistochemistry: Calreticulin-2/CALR3 Antibody [NBP2-33390]
Immunohistochemistry: Calreticulin-2/CALR3 Antibody [NBP2-33390] - Examples of immunohistochemical staining for Calreticulin-2/CALR3 (antibody NBP2-33390) in normal and affected tissues. No expression in myocardial tissue from (a) patient with c.564del-positive DCM, b patient with ischemic cardiomyopathy, and c child, d newborn, and e fetus with structurally normal hearts. Instead, we observed positive staining of arteriolar smooth muscle cells in all samples (arrows). f High expression of CALR3 in testicular germ cells as positive control tissue. Scale bars: 1 mm Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41431-018-0208-1), licensed under a CC-BY license.Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390]
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390] - Staining of human testis shows strong cytoplasmic positivity in spermatids.Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390]
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33390] - Staining of human endometrium shows low expression as expected.Applications for Calreticulin-2/CALR3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:5000 - 1:10000
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Calreticulin-2
Additional Calreticulin-2 Products
Product Documents for Calreticulin-2/CALR3 Antibody
Product Specific Notices for Calreticulin-2/CALR3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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