Calreticulin-2/CALR3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33524

Novus Biologicals, part of Bio-Techne
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NBP2-33524
NBP2-33524-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calreticulin-2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SIEYDWNLTSLKKETSPAESKDWEQTKDNKAQDWEKHFLDASTSKQSDWNGDLDGDWPAPMLQKPPYQDGLKPEGIHKGVWLHRKMKNTDYLTQYDLSEF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calreticulin-2/CALR3 Antibody

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Staining in human testis and endometrium tissues using anti-CALR3 antibody. Corresponding CALR3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Staining of human colon, kidney, liver and testis using Anti-CALR3 antibody NBP2-33524 (A) shows similar protein distribution across tissues to independent antibody NBP2-33390 (B).
Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524]

Immunohistochemistry-Paraffin: Calreticulin-2/CALR3 Antibody [NBP2-33524] - Immunohistochemical staining of CT antigens in normal tissues. Among 22 normal tissues (see materials and methods), ADAM2, CALR3 (shown in image), SAGE1 and MAGE-A proteins were only detected in the germ cells of the testis. More specifically, SAGE-1 and MAGE-A were found in spermatogonia, while ADAM2 and CALR3 were present in post-meiotic germ cells. Only MAGE-A proteins were detected in placenta (all pictures magnification x20). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0134967), licensed under a CC-BY license.
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Applications for Calreticulin-2/CALR3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calreticulin-2

Calreticulins, such as CALR3, are Ca(2+)-binding chaperones localized mainly in the endoplasmic/sarcoplasmic reticulum Persson et al. (2002) [PubMed 12384296].[supplied by OMIM]

Alternate Names

CALR3, Calreticulin2, Crt2

Gene Symbol

CALR3

UniProt

Q96L12

Additional Calreticulin-2 Products

Product Documents for Calreticulin-2/CALR3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calreticulin-2/CALR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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