CaM Kinase II alpha Antibody (6G9) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1983B

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Xenopus

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockdown Validated

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 6G9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CaM Kinase II alpha Antibodies »

Product Specifications

Immunogen

Partially purified rat CaMKII

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28421537). Xenopus reactivity reported in scientific literature (PMID: 29412139). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm , Mitochondrion , Nucleus , Cell Junction , Synapse , Presynaptic Cell Membrane

Specificity

Detects approx 50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CaM Kinase II alpha Antibody (6G9) [Biotin]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CaM Kinase II alpha

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Entrez Gene IDs

815 (Human); 12322 (Mouse); 25400 (Rat)

Gene Symbol

CAMK2A

UniProt

P11275 (Rat)

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha Antibody (6G9) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CaM Kinase II alpha Antibody (6G9) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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