CaM Kinase II alpha [p Thr286] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-184
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Rabbit
Cited:
Human
Predicted:
Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH. Accession # P11275
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 28972541).
Modification
p Thr286
Localization
Cell membrane; presynaptic membrane. Cell junction; synapse.
Specificity
Specific for endogenous levels of the ~50 kDa CaM Kinase II alpha and the ~60 kDa beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CaM Kinase II alpha [p Thr286] Antibody
![Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NB300-184]](https://resources.bio-techne.com/images/products/CaMKII-alpha-[p-Thr286]-Antibody-Western-Blot-NB300-184-img0003.jpg "Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NB300-184]")
Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NB300-184]
Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with lambda-Ptase.Applications for CaM Kinase II alpha [p Thr286] Antibody
Application
Recommended Usage
Western Blot
1:1000
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28972541).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 7 reviews rated 4.9 using NB300-184 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CaM Kinase II alpha
Long Name
Calcium/Calmodulin-dependent Protein Kinase II alpha
Alternate Names
CAMK2A
Gene Symbol
CAMK2A
UniProt
Additional CaM Kinase II alpha Products
Product Documents for CaM Kinase II alpha [p Thr286] Antibody
Product Specific Notices for CaM Kinase II alpha [p Thr286] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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