CaM Kinase II alpha [p Thr286] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13299

Novus Biologicals, part of Bio-Techne
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NBP3-13299
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Chicken (100%), Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CaM Kinase II alpha Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr286 of human CaM Kinase II alpha. The exact sequence is proprietary.

Modification

p Thr 286

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for CaM Kinase II alpha [p Thr286] Antibody

Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NBP3-13299]

Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NBP3-13299]

Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NBP3-13299] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II alpha (phospho Thr286) antibody (NBP3-13299) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for CaM Kinase II alpha [p Thr286] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaM Kinase II alpha

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Gene Symbol

CAMK2A

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha [p Thr286] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CaM Kinase II alpha [p Thr286] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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