cAMP Antibody (RM466)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18822
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Non-species specific
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM466
Concentration
1 mg/ml
Product Summary for cAMP Antibody (RM466)
Immunogen
KLH-conjugated cAMP
Specificity
This antibody reacts to cAMP. No cross reactivity with other cyclic nucleotide or nucleoside phosphate.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for cAMP Antibody (RM466)
ELISA: cAMP Antibody (RM466)[NBP3-18822]
ELISA: cAMP Antibody (RM466)[NBP3-18822]Competitive ELISA of cAMP using anti-cAMP (RM466). The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-cAMP(RM466) (50 µl/well) was added and incubated. After wash and block, different concentrations of cAMP (25 µl/well) were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cAMP. (71-1358-00) TMB was used to develop the color after incubation and wash.Applications for cAMP Antibody (RM466)
Application
Recommended Usage
ELISA
0.05 - 0.5 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: cAMP
Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.
Long Name
Cyclic AMP
Alternate Names
Cyclic AMP
Additional cAMP Products
Product Documents for cAMP Antibody (RM466)
Product Specific Notices for cAMP Antibody (RM466)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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