Canine IL-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1964
Key Product Details
Validated by
Biological Validation
Species Reactivity
Canine
Applications
Immunocytochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant canine IL-5
Phe20-Ser134
Accession # Q95J76
Phe20-Ser134
Accession # Q95J76
Specificity
Detects canine canine IL‑5 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Canine IL-5 Antibody
IL‑5 in Canine PBMCs.
IL-5 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Canine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by IL-5 and Neutralization by Canine IL-5 Antibody.
Recombinant Canine IL-5 (1964-CL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-5 (30 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964). The ND50 is typically 0.75-3.0 µg/mL.Applications for Canine IL-5 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA
Sample: Immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA
Western Blot
0.1 µg/mL
Sample: Recombinant Canine IL-5 (Catalog # 1964-CL)
Sample: Recombinant Canine IL-5 (Catalog # 1964-CL)
Neutralization
Measured by its ability to neutralize IL-5-induced proliferation in the TF-1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 30 ng/mL Recombinant Canine IL-5.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5
Interleukin-5 (IL-5) is a 40‑45 kDa secreted disulfide-linked homodimeric glycoprotein that plays an important role in the differentiation, growth, and function of eosinophils. It also primes basophils for histamine and leukotriene release. In mice, IL-5 also induces the proliferation, differentiation, and immunoglobulin production of B cells especially B-1 cells that constitutively express IL-5 receptor alpha. IL-5 is primarily produced by CD4+ Th2 cells. Other cell types, including mast cells, visceral smooth muscle cells, bronchial epithelium, CD16+ NK cells, eosinophils and gamma delta T cells, can also produce IL-5. Canine IL-5 is synthesized as a 134 amino acid (aa) precursor that contains a 21 aa signal sequence and a 113 aa mature segment. Mature canine IL-5 shares 62%, 66%, 85%, 84%, 58%, and 56% aa sequence identity with mature human, guinea pig, porcine, feline, mouse, and rat IL-5, respectively. The receptor for IL-5 consists of a 60 kDa ligand-binding subunit (IL-5 R alpha) and a 120 kDa signal-transducing subunit ( betac) (1‑7).
References
- Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
- Karlen, S. et al. (1998) Intern. Rev. Immunol. 16:227.
- Takatsu, K. (1998) Cytokine Growth Factor Rev. 9:25.
- Zabeau, L. et al. (2003) Curr. Drug Targets Inflamm. Allergy 2:319.
- Yang, S. et al. (2001) J. Interferon Cytokine Res. 21:361.
- Jans, D.A. and G. Hassan (1998) BioEssays 20:400.
- Geijsen, N. et al. (2001) Cytokine Growth Factor Rev. 12:19.
Long Name
Interleukin 5
Alternate Names
BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF
Entrez Gene IDs
Gene Symbol
IL5
UniProt
Additional IL-5 Products
Product Documents for Canine IL-5 Antibody
Product Specific Notices for Canine IL-5 Antibody
For research use only
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