Cannabinoid R1/CB1/CNR1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2427
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Predicted:
Canine (100%), Human (100%). Backed by our 100% Guarantee.
Applications
Validated:
Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-HTSEDGKVQVTRPDQ corresponding to internal region according to NP_001153732.1, NP_001153731.1, NP_001153730.1, NP_001153698.1, NP_149421.1.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24291518).
Specificity
This antibody is expected to recognize both reported isoforms. Isoform a (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1) and isoform b (NP_149421.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Cannabinoid R1/CB1/CNR1 Antibody
![Western Blot: Cannabinoid R1/CB1/CNR1 Antibody [NB100-2427]](https://resources.bio-techne.com/images/products/Cannabinoid-R1-CB1-CNR1-Antibody-Western-Blot-NB100-2427-img0001.jpg "Western Blot: Cannabinoid R1/CB1/CNR1 Antibody [NB100-2427]")
Western Blot: Cannabinoid R1/CB1/CNR1 Antibody [NB100-2427]
Western Blot: Cannabinoid R1/CB1/CNR1 Antibody [NB100-2427] - Western blot analysis of HeLa lysate non-glycosylated (A) + peptide (B) with antibody at 1 ug/mL and HeLa lysate glycosylated (C) + peptide (D) with antibody at 0.5 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.Applications for Cannabinoid R1/CB1/CNR1 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
0.5 - 2 ug/mL
Application Notes
Western blot: Approx 55 kDa (non-glycosylated) and 65 kDa (glycosylated) bands observed in lysates of cell line HeLa (calculated MW of 52.9 kDa according to NP_057167.2;). Both bands were successfully blocked by incubation with the immunizing peptide,
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cannabinoid R1/CB1/CNR1
Long Name
Cannabinoid Receptor 1
Alternate Names
CANN6, CannabinoidR1, CB-R, CB1, CB1A, CB1K5, CB1R, CNR1, SKR6R
Gene Symbol
CNR1
UniProt
Additional Cannabinoid R1/CB1/CNR1 Products
Product Documents for Cannabinoid R1/CB1/CNR1 Antibody
Product Specific Notices for Cannabinoid R1/CB1/CNR1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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