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Carbonic Anhydrase I/CA1 Antibody [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-942

Novus Biologicals, part of Bio-Techne
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NB600-942-0.1mg

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

Product Summary for Carbonic Anhydrase I/CA1 Antibody [HRP]

Immunogen

Carbonic Anhydrase I/CA1/CA1 [Human Erythrocytes] (Uniprot: P00915)

Reactivity Notes

Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.

Localization

Cytoplasmic

Specificity

Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ]

Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody [HRP]

Western Blot: Carbonic Anhydrase I/CA1 Antibody [HRP] [NB600-942] - Western Blot of Goat Carbonic Anhydrase I/CA1/CA1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight.Lane 2: Carbonic Anhydrase I/CA1 [10ug]. Primary antibody: Anti-Carbonic Anhydrase I/CA1 HRP Antibody at 1:1000 for 1 hr at RT.Secondary antibody: Streptavidin-HRP antibody at 1:40,000 for 30mins at RT.Blocking bufferPredicted/Observed size: ~29kDa.
Carbonic Anhydrase I/CA1 Antibody [HRP]

Carbonic Anhydrase I/CA1 Antibody [HRP]

Western Blot of Carbonic Anhydrase I/CA1 Antibody [HRP]. Lane 1: Opal Prestained Molecular Weight

Applications for Carbonic Anhydrase I/CA1 Antibody [HRP]

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunohistochemistry

1:500-1:2500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000-1:5000
Application Notes
This product is tested by ELISA and western blot and useful in immunohistochemistry. Optimal titers should be obtained by researchers.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase I/CA1

Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.

Alternate Names

CA1

Gene Symbol

CA1

UniProt

Additional Carbonic Anhydrase I/CA1 Products

Product Documents for Carbonic Anhydrase I/CA1 Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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