Carbonic Anhydrase I/CA1 Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-942
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Summary for Carbonic Anhydrase I/CA1 Antibody [HRP]
Immunogen
Carbonic Anhydrase I/CA1/CA1 [Human Erythrocytes] (Uniprot: P00915)
Reactivity Notes
Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.
Localization
Cytoplasmic
Specificity
Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ]
Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ]
Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody [HRP]
Western Blot: Carbonic Anhydrase I/CA1 Antibody [HRP] [NB600-942] - Western Blot of Goat Carbonic Anhydrase I/CA1/CA1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight.Lane 2: Carbonic Anhydrase I/CA1 [10ug]. Primary antibody: Anti-Carbonic Anhydrase I/CA1 HRP Antibody at 1:1000 for 1 hr at RT.Secondary antibody: Streptavidin-HRP antibody at 1:40,000 for 30mins at RT.Blocking bufferPredicted/Observed size: ~29kDa.
Carbonic Anhydrase I/CA1 Antibody [HRP]
Western Blot of Carbonic Anhydrase I/CA1 Antibody [HRP]. Lane 1: Opal Prestained Molecular WeightApplications for Carbonic Anhydrase I/CA1 Antibody [HRP]
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunohistochemistry
1:500-1:2500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000-1:5000
Application Notes
This product is tested by ELISA and western blot and useful in immunohistochemistry. Optimal titers should be obtained by researchers.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase I/CA1
Additional Carbonic Anhydrase I/CA1 Products
Product Documents for Carbonic Anhydrase I/CA1 Antibody [HRP]
Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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