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Carbonic Anhydrase I/CA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-6619

Novus Biologicals, part of Bio-Techne
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NB120-6619-0.1mg

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Carbonic Anhydrase I/CA1/CA1 [Human Erythrocytes] (Uniprot: P00915)

Reactivity Notes

Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.

Localization

Cytoplasmic

Specificity

Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody

Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]

Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]

Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Lane 1: Carbonic Anhydrase I. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase I antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase I.
Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]

Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]

Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Tissue: spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated at 5 ug/mL for 1 h at RT.
Carbonic Anhydrase I/CA1 Antibody

Carbonic Anhydrase I/CA1 Antibody

Immunohistochemistry of Carbonic Anhydrase I/CA1 Antibody. Tissue: human spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Carbonic Anhydrase I antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Staining: Carbonic Anhydrase 1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Applications for Carbonic Anhydrase I/CA1 Antibody

Application
Recommended Usage

ELISA

1:2000-1:20000

Immunohistochemistry

10ug/ml

Western Blot

1:500-1:2000
Application Notes
This product has been tested in western blot and immunohistochemistry and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase I/CA1

Carbonic anhydrase performs an important role in respiration by facilitating transfer of CO2. Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.

Alternate Names

CA1

Entrez Gene IDs

759 (Human)

Gene Symbol

CA1

UniProt

Additional Carbonic Anhydrase I/CA1 Products

Product Documents for Carbonic Anhydrase I/CA1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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