Carbonic Anhydrase IX/CA9 Antibody (RM486)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23472
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM486
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Carbonic Anhydrase IX/CA9 Antibody (RM486)
Immunogen
A peptide corresponding to the C-terminus of human Carbonic Anhydrase IX/CA9 (Uniprot#: Q16790)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Carbonic Anhydrase IX/CA9 Antibody (RM486)
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] -
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] - Immunohistochemical staining of formalin fixed and paraffin embedded human stomach tissue sections using Clone RM486) at 1:100 dilution.
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] -
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] - Immunohistochemical staining of formalin fixed and paraffin embedded human liver tissue sections using Clone RM486) at 1:100 dilution.
Western Blot: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] -
Western Blot: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] - Western Blot of SW620 cell lysate using Clone RM486) at 1:2500 dilution.Applications for Carbonic Anhydrase IX/CA9 Antibody (RM486)
Application
Recommended Usage
Immunohistochemistry
1:100 -1:200
Western Blot
1:1000 - 1:2500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase IX/CA9
Carbonic anhydrase IX (theoretical molecular weight 50kDa) belongs to the monomeric alpha class and is a single pass-transmembrane protein with two extracellular domains which serve catalytic and cell adhesion functions (2, 3). By cooperating with sodium bicarbonate cotransporters (NBC), lactate and proton exporting monocarboxylic acid transporters (MCT), and a sodium/hydrogen exchanger (NHE), carbonic anhydrase IX is involved in pH regulation across the cell membrane. This functional property protects cancer cells from intracellular acidification and partly explains the role of carbonic anhydrase IX in cancer cell survival and proliferation. In contrast, the pH regulating activity of carbonic anhydrase IX induces extracellular acidification, which has been implicated in epithelial to mesenchymal transition (EMT) and promoting cancer invasion. Carbonic anhydrase IX is frequently overexpressed in cancer cells (e.g., colorectal-, breast-, lung-carcinoma and brain tumors), an effect promoted by hypoxia within the tumor microenvironment (4). An exception are tumors carrying pVHL inactivating mutations, such as clear cell renal cell carcinoma (ccRCC), where HIF-alpha is stabilized due to dysfunctional proteasomal targeting and can induce HRE (Hypoxia Response Element) containing genes even under physiological normoxia (5). Carbonic anhydrase IX may be detected by immunostaining in tumors, which is found in association with necrotic tissue and metastatic cells. Because the expression of carbonic anhydrase IX correlates with both tumor grade and stage, analysis of its expression in tumors serves as a prognostic factor (4, 6).
References
1. Tripp, B. C., Smith, K., & Ferry, J. G. (2001). Carbonic Anhydrase: New Insights for an Ancient Enzyme. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.R100045200
2. Nishimori, I., & Onishi, S. (2001). Carbonic anhydrase isozymes in the human pancreas. Digestive and Liver Disease. https://doi.org/10.1016/s1590-8658(01)80138-9
3. Zavadova, Z., & Zavada, J. (2005). Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment. Oncology Reports. https://doi.org/10.3892/or.13.5.977
4. Pastorekova, S., & Gillies, R. J. (2019). The role of carbonic anhydrase IX in cancer development: links to hypoxia, acidosis, and beyond. Cancer and Metastasis Reviews. https://doi.org/10.1007/s10555-019-09799-0
5. Haase, V. (2009). The VHL Tumor Suppressor: Master Regulator of HIF. Current Pharmaceutical Design. https://doi.org/10.2174/138161209789649394
6. Young, J. R., Coy, H., Kim, H. J., Douek, M., Sisk, A., Pantuck, A. J., & Raman, S. S. (2018). Association of the gross appearance of intratumoral vascularity at MDCT with the carbonic anhydrase IX score in clear cell renal cell carcinoma. American Journal of Roentgenology. https://doi.org/10.2214/AJR.18.19725
Alternate Names
CA9, G250, MN, RCC
Gene Symbol
CA9
Additional Carbonic Anhydrase IX/CA9 Products
Product Documents for Carbonic Anhydrase IX/CA9 Antibody (RM486)
Product Specific Notices for Carbonic Anhydrase IX/CA9 Antibody (RM486)
[Public Notes]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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