Carbonic Anhydrase XII/CA12 Antibody (CL0278)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52901

Novus Biologicals, part of Bio-Techne
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NBP2-52901
NBP2-52901-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0278

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Carbonic Anhydrase XII/CA12 Antibodies »

Product Summary for Carbonic Anhydrase XII/CA12 Antibody (CL0278)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQ

Epitope

HLQHVKYKGQEAFVP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Carbonic Anhydrase XII/CA12 Antibody (CL0278)

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0278) [NBP2-52901]

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0278) [NBP2-52901]

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0278) [NBP2-52901] - Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0278) [NBP2-52901]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0278) [NBP2-52901]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0278) [NBP2-52901] - Staining of human stomach shows moderate membranous immunoreactivity in glandular cells.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0278) [NBP2-52901]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0278) [NBP2-52901]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0278) [NBP2-52901] - Staining of human kidney shows strong membranous immunoreactivity in renal tubules, but not glomeruli.
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Applications for Carbonic Anhydrase XII/CA12 Antibody (CL0278)

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase XII/CA12

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.

Alternate Names

CA12

Gene Symbol

CA12

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Carbonic Anhydrase XII/CA12 Antibody (CL0278)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Carbonic Anhydrase XII/CA12 Antibody (CL0278)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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