Carbonic Anhydrase XII/CA12 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81668
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQ
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%). Reactivity reported in scientific literature (PMID: 22016510)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Carbonic Anhydrase XII/CA12 Antibody
![Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-XII-CA12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81668-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]")
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]
Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668] - Staining of human cell line RT4 shows localization to nucleus. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-XII-CA12-Antibody-Immunohistochemistry-Paraffin-NBP1-81668-img0008.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]")
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.Applications for Carbonic Anhydrase XII/CA12 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
Reported in (PMID: 26911677).
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase XII/CA12
Alternate Names
CA12
Gene Symbol
CA12
Additional Carbonic Anhydrase XII/CA12 Products
Product Documents for Carbonic Anhydrase XII/CA12 Antibody
Product Specific Notices for Carbonic Anhydrase XII/CA12 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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