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Carboxylesterase 2/CES2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32653

Novus Biologicals, part of Bio-Techne
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NBP1-32653

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Carboxylesterase 2/CES2. The exact sequence is proprietary.

Localization

Endoplasmic reticulum lumen

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Carboxylesterase 2/CES2 Antibody

Western Blot: Carboxylesterase 2/CES2 Antibody [NBP1-32653]

Western Blot: Carboxylesterase 2/CES2 Antibody [NBP1-32653]

Western Blot: CES2 Antibody [NBP1-32653] - Sample (30 ug of whole cell lysate)A: Hep G2 7. 5% SDS PAGE diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Carboxylesterase 2/CES2 Antibody [NBP1-32653]

Immunocytochemistry/ Immunofluorescence: Carboxylesterase 2/CES2 Antibody [NBP1-32653]

Immunocytochemistry/Immunofluorescence: CES2 Antibody [NBP1-32653] - Sample: HepG2 cells were fixed in iced-cold MeOH for 5 min. Green: CES2 protein stained by CES2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Carboxylesterase 2/CES2 Antibody [NBP1-32653]

Immunohistochemistry-Paraffin: Carboxylesterase 2/CES2 Antibody [NBP1-32653]

Immunohistochemistry-Paraffin: CES2 Antibody [NBP1-32653] - Paraffin-embedded colon; antibody dilution 1:500.

Applications for Carboxylesterase 2/CES2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Carboxylesterase 2/CES2

Carboxylesterase 2 is a member of a large multigene family. The enzymes encoded by these genes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolize long-chain fatty acid esters and thioesters. The specific function of this enzyme has not yet been determined; however, it is speculated that carboxylesterases may play a role in lipid metabolism and/or the blood-brain barrier system. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

CE-2, CES2, PCE-2

Gene Symbol

CES2

Additional Carboxylesterase 2/CES2 Products

Product Documents for Carboxylesterase 2/CES2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carboxylesterase 2/CES2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.