CARD11/CARMA1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1200
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QRKTIWVDEDQL corresponding to C-Terminus according to NP_115791.3.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24036998).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for CARD11/CARMA1 Antibody
Western Blot: CARD11/CARMA1 Antibody [NB100-1200]
Western Blot: CARD11/CARMA1 Antibody [NB100-1200] - Staining of Human Jurkat cells (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody [NB100-1200]
Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody [NB100-1200] - Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for CARD11/CARMA1 Antibody
Application
Recommended Usage
Immunohistochemistry
2 - 5 ug/ml
Immunohistochemistry-Paraffin
2-5 ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. IHC: Paraffin embedded human spleen shows nuclear staining.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CARD11/CARMA1
Long Name
Caspase Recruitment Domain-containing Protein 11
Alternate Names
BIMP3, CARMA1
Gene Symbol
CARD11
UniProt
Additional CARD11/CARMA1 Products
Product Documents for CARD11/CARMA1 Antibody
Product Specific Notices for CARD11/CARMA1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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