Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all CARD11/CARMA1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1. The immunogen is located within the last 50 amino acids of CARMA1. Amino Acid Squence: EEQRKTIWVDEDQL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARD11/CARMA1 Antibody - BSA Free

Western Blot: CARD11/CARMA1 AntibodyBSA Free [NBP1-77219]

Western Blot: CARD11/CARMA1 AntibodyBSA Free [NBP1-77219]

Western Blot: CARD11/CARMA1 Antibody [NBP1-77219] - Western blot analysis of CARD11 expression in mouse thymus cell lysate with anti-CARMA1 using antibody at 0.5 (lane A), 1 (lane B), and 2 ug /ml (lane C), respectively.
CARD11/CARMA1 Antibody - BSA Free

Immunohistochemistry: CARD11/CARMA1 Antibody - BSA Free [NBP1-77219] -

Immunohistochemistry: CARD11/CARMA1 Antibody - BSA Free [NBP1-77219] - Immunohistochemistry of CARD11/CARMA1 in mouse thymus with CARD11/CARMA1 antibody at 10 u/mL.
CARD11/CARMA1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CARD11/CARMA1 Antibody - BSA Free [NBP1-77219] -

Immunocytochemistry/ Immunofluorescence: CARD11/CARMA1 Antibody - BSA Free [NBP1-77219] - Immunofluorescence of CARD11/CARMA1 in Mouse Spleen cells with CARD11/CARMA1 antibody at 20 ug/mL.

Applications for CARD11/CARMA1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
A band at approximately 125 kDa can be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARD11/CARMA1

CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IkappaK complex, thereby regulating activation of the NF-kappaB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.

Long Name

Caspase Recruitment Domain-containing Protein 11

Alternate Names

BIMP3, CARMA1

Gene Symbol

CARD11

UniProt

Q9BXL7 (Human)

Additional CARD11/CARMA1 Products

Product Documents for CARD11/CARMA1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CARD11/CARMA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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