CARD12 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-225
![Western Blot: CARD12 AntibodyBSA Free [NB500-225]](https://resources.bio-techne.com/images/products/CARD12-Antibody-Western-Blot-NB500-225-img0002.jpg "Western Blot: CARD12 AntibodyBSA Free [NB500-225]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
N terminus (MNFIKDNSRALIQRMGM) of the A and B isoforms of the human CLAN protein.
Reactivity Notes
This antibody reacts with human CLAN.
Localization
Cytoplasmic filaments
Specificity
Reacts with the N terminus (MNFIKDNSRALIQRMGM) of the A and B isoforms of the human CLAN protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CARD12 Antibody - BSA Free
Western Blot: CARD12 AntibodyBSA Free [NB500-225]
Western Blot: CARD12 Antibody [NB500-225] - Immunoblot analysis of cN-II and Ipaf (CARD12) protein expression in different human cell lines. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0121525) licensed under a CC-BY license.![Western Blot: CARD12 AntibodyBSA Free [NB500-225]](https://resources.bio-techne.com/images/products/CARD12-Antibody-Western-Blot-NB500-225-img0001.jpg "Western Blot: CARD12 AntibodyBSA Free [NB500-225]")
Western Blot: CARD12 AntibodyBSA Free [NB500-225]
Western Blot: CARD12 Antibody [NB500-225] - Western Blot analysis of CLAN in lung lysate with anti-CLAN at a 1:500 dilution. Detects CLAN-A and CLAN-B isoforms.
Western Blot: CARD12 Antibody - BSA Free [NB500-225] -
In vitro interaction between cN-II and Ipaf B.After loading Ni-talon beads with E. coli “BL21(DE3)” crude extract without (■) or with cN-II (●), beads were washed and E. coli “BL21 Rosetta(DE3)” extracts containing Ipaf B were loaded. Elution buffer containing 250 mM imidazole was used from fraction 21 to detach His-tagged cN-II and elute proteins. A) proteins elution profile; B) immunoblotting with CARD12 and cN-II antibodies on elution fractions 3, 22, 24, 25 and 26 from chromatography.Applications for CARD12 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500-1:1000
Application Notes
This antibody is useful for western blot analysis where bands are seen around 110 kDa and 40 kDa representing isoforms A and B respectively.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CARD12
Alternate Names
CARD12leucine rich repeat and CARD domaincontaining 4, caspase recruitment domain family, member 12, Caspase recruitment domain-containing protein 12, Clan protein, CLAN1ipaf, CLANCARD, LRR, and NACHT-containing protein, CLR2.1, Ice protease-activating factor, Ipaf, NLR family, CARD domain containing 4
Entrez Gene IDs
58484 (Human)
Gene Symbol
NLRC4
Additional CARD12 Products
Product Documents for CARD12 Antibody - BSA Free
Product Specific Notices for CARD12 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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