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CARD9 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76679

Novus Biologicals, part of Bio-Techne
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NBP1-76679
NBP1-76679-0.025mg

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CARD9. The immunogen is located within the last 50 amino acids of CARD9. Amino Acid Squence: DRENTTGSDNTDDTEGS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CARD9 Antibody - BSA Free

Western Blot: CARD9 AntibodyBSA Free [NBP1-76679]

Western Blot: CARD9 AntibodyBSA Free [NBP1-76679]

Western Blot: CARD9 Antibody [NBP1-76679] - Analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 ug /ml.
Immunocytochemistry/ Immunofluorescence: CARD9 Antibody - BSA Free [NBP1-76679]

Immunocytochemistry/ Immunofluorescence: CARD9 Antibody - BSA Free [NBP1-76679]

Immunocytochemistry/Immunofluorescence: CARD9 Antibody [NBP1-76679] - K562 cells with CARD9 antibody at 10 ug/ml.
CARD9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CARD9 Antibody - BSA Free [NBP1-76679] -

Immunocytochemistry/ Immunofluorescence: CARD9 Antibody - BSA Free [NBP1-76679] - Immunocytochemistry of CARD9 in K562 cells with CARD9 antibody at 10 u/mL.

Applications for CARD9 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

2.5 ug/ml
Application Notes
A band at approximately 59 kDa can be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARD9

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10. CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-kappaB. CARD9 is an upstream activator of BCL-10 and NF-kappaB signaling.

Long Name

Caspase Recruitment Domain Containing Protein 9

Alternate Names

CANDF2, caspase recruitment domain family, member 9, caspase recruitment domain-containing protein 9, hCARD9

Gene Symbol

CARD9

Additional CARD9 Products

Product Documents for CARD9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CARD9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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