CARD9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30275

Novus Biologicals, part of Bio-Techne
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NBP3-30275
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CARD9 Antibodies »

Product Specifications

Immunogen

between 450 and 500

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARD9 Antibody

CARD9 Antibody

Immunoprecipitation:CARD9 AntibodyNBP3-30275] -

Immunoprecipitation:CARD9 AntibodyNBP3-30275] -Detection of human CARD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SW620 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARD9 antibody (NBP3-30275 lot 2) used for IP at 1 µg per reaction. CARD9 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30275-1). For blotting immunoprecipitated CARD9, NBP3-30275 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for CARD9 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CARD9

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10. CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-kB. CARD9 is an upstream activator of BCL-10 and NF-kB signaling.

Long Name

Caspase Recruitment Domain Containing Protein 9

Alternate Names

CANDF2, caspase recruitment domain family, member 9, caspase recruitment domain-containing protein 9, hCARD9

Gene Symbol

CARD9

Additional CARD9 Products

Product Documents for CARD9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CARD9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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