Cardiac Troponin T Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13391

Novus Biologicals, part of Bio-Techne
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NBP3-13391
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%), Feline (100%), Guinea Pig (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Troponin T Antibodies »

Product Summary for Cardiac Troponin T Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Cardiac Troponin T. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Cardiac Troponin T Antibody

Western Blot: Cardiac Troponin T Antibody [NBP3-13391]

Western Blot: Cardiac Troponin T Antibody [NBP3-13391]

Western Blot: Cardiac Troponin T Antibody [NBP3-13391] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13391) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391]

Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391]

Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391] - Rat heart. Cardiac Troponin T stained by Cardiac Troponin T antibody (NBP3-13391) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Cardiac Troponin T Antibody [NBP3-13391]

Western Blot: Cardiac Troponin T Antibody [NBP3-13391]

Western Blot: Cardiac Troponin T Antibody [NBP3-13391] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (NBP3-13391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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Applications for Cardiac Troponin T Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin T

Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.

Alternate Names

CMH2, CMPD2, cTnT, TNNT2, TnTC

Gene Symbol

TNNT2

Additional Troponin T Products

Product Documents for Cardiac Troponin T Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cardiac Troponin T Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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