CARF/CDKN2AIP Antibody (1F7)
Novus Biologicals, part of Bio-Techne | Catalog # H00055602-M06
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1F7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CARF (AAH22270, 1 a.a. ~ 579 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQEVSEYLSQNPRVAAWVEALRCDGETDKHWRHRRDFLLRNAGDLAPAGGAASASTDEAADAESGTRNRQLQQLISFSMAWANHVFLGCRYPQKVMDKILSMAEGIKVTDAPTYTTRDELVAKVKKRGISSSNEGVEEPSKKRVIEGKNSSAVEQDHAKTSAKTERASAQQENSSTCIGSAIKSESGNSARSSGISSQNSSTSDGDRSVSSQSSSSVSSQVTTAGSGKASEAEAPDKHGSSFVSLLKSSVNSHMTQSTDSRQQSGSPKKSALEGSSASASQSSSEIEVPLLGSSGSSEVELPLLSSKPSSETASSGLTSKTSSEASVSSSVAKNSSSSGTSLLTPKSSSSTNTSLLTSKSTSQVAASLLASKSSSQTSGSLVSKSTSLASVSQLASKSSSQTSTSQLPSKSTSQSSESSVKFSCKLTNEDVKQKQPFFNRLYKTVAWKLVAVGGFSPNVNHGELLNAAIEALKATLDVFFVPLKELADLPQNKSSQESIVCELRCKSVYLGTGCGKSKENAKAVASREALKLFLKKKVVVKICKRKYRGSEIEDLVLLDEESRPVNLPPALKHPQELL
Reactivity Notes
Human. Other species not tested.
Specificity
CARF - collaborates/cooperates with ARF (alternate reading frame) protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CARF/CDKN2AIP Antibody (1F7)
![Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06]](https://resources.bio-techne.com/images/products/CARF-CDKN2AIP-Antibody-1F7-Western-Blot-H00055602-M06-img0004.jpg "Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06]")
Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06]
Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06] - Analysis of CARF expression in transfected 293T cell line by CARF monoclonal antibody (M06), clone 1F7. Lane 1: CARF transfected lysatE (61.1 KDa). Lane 2: Non-transfected lysate.![Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06]](https://resources.bio-techne.com/images/products/CARF-CDKN2AIP-Antibody-1F7-Western-Blot-H00055602-M06-img0002.jpg "Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06]")
Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06]
Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06] - Western blot analysis of CARF over-expressed 293 cell line. Lane 2 cotransfected with CARF Validated Chimera RNAi or non-transfected control. Blot probed with H00055602-M06. GAPDH ( 36.1 kDa ) used as loading control.![ELISA: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06]](https://resources.bio-techne.com/images/products/CARF-CDKN2AIP-Antibody-1F7-ELISA-H00055602-M06-img0003.jpg "ELISA: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06]")
ELISA: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06]
ELISA: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06] - Detection limit for recombinant GST tagged CARF is approximately 0.1ng/ml as a capture antibody.Applications for CARF/CDKN2AIP Antibody (1F7)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CARF
Long Name
Collaborates/Cooperates With ARF [Alternate Reading Frame] Protein
Alternate Names
CDKN2AIP
Entrez Gene IDs
55602 (Human)
Gene Symbol
CDKN2AIP
UniProt
Additional CARF Products
Product Documents for CARF/CDKN2AIP Antibody (1F7)
Product Specific Notices for CARF/CDKN2AIP Antibody (1F7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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