Casein Kinase 1 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50030

Novus Biologicals, part of Bio-Techne
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NBP2-50030
NBP2-50030SS
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Chicken, Equine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Casein Kinase 1 alpha Antibodies »

Product Specifications

Immunogen

Recombinant full length Casein Kinase 1 alpha (shortest isoform). [UniProt# P48729]

Specificity

This antibody will bind to bind to all Casein Kinase 1 alpha isoforms.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Casein Kinase 1 alpha Antibody

Western Blot: Casein Kinase 1 alpha Antibody [NBP2-50030]

Western Blot: Casein Kinase 1 alpha Antibody [NBP2-50030]

Western Blot: Casein Kinase 1 alpha Antibody [NBP2-50030] - Analysis of whole rat spinal cord homogenate stained with NBP2-50030, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~38kDa and a less prominent band at ~34kDa corresponds to other Casein Kinase 1 alpha isotypes.
Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 alpha Antibody [NBP2-50030]

Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 alpha Antibody [NBP2-50030]

Immunocytochemistry/Immunofluorescence: Casein Kinase 1 alpha Antibody [NBP2-50030] - HeLa cells stained with NBP2-50030 (red) and panspecific mouse monoclonal antibody to nuclear pore complexes NBP1-05390 (green). Casein Kinase 1 alpha has a particulate distribution both in the nucleus and the cytoplasm. Blue is the Hoechst DNA stain.

Applications for Casein Kinase 1 alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Immunohistochemistry

1:5000

Western Blot

1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

PBS. Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Casein Kinase 1 alpha

Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Ialpha, Igamma, Idelta and Iepisilon, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The a catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Alternate Names

CSNK1A1, HLCDGP1

Gene Symbol

CSNK1A1

UniProt

P48729 (Human)

Additional Casein Kinase 1 alpha Products

Product Documents for Casein Kinase 1 alpha Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Casein Kinase 1 alpha Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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