Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Chicken (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation (Negative), Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Casein Kinase 2 alpha Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB200-171, NB100-97831

Scientific Data Images for Casein Kinase 2 alpha Antibody

Western Blot: Casein Kinase 2 alpha Antibody [NB100-376]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-376]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Casein Kinase 2 alpha Antibody [NB100-376]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-376]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 ug/ml.

Applications for Casein Kinase 2 alpha Antibody

Application
Recommended Usage

Western Blot

1:1000-1:5000
Application Notes
Not recommended for immunoprecipitation (use NB 100-378 for IP). WB of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Casein Kinase 2 alpha

CKII alpha is involved in leukemogensis and tumorigenesis. It is a catalytic, ubiquitous cellular kinase that can use ATP and GTP as phosphorylation donors. CKII alpha also plays a role in many proliferation-related processes in the cell.

Alternate Names

CK2A1, CSNK2A1

Entrez Gene IDs

1457 (Human)

Gene Symbol

CSNK2A1

UniProt

NP_001886.1 (Human)

Additional Casein Kinase 2 alpha Products

Product Documents for Casein Kinase 2 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Casein Kinase 2 alpha Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB200-171, NB100-97831

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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