Caspase-1 Antibody - (cleaved)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13233
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Caspase-1 Antibody - (cleaved)
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Caspase-1 (cleaved Asp297) . The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Caspase-1 Antibody - (cleaved)
Western Blot: Caspase-1 Antibody(cleaved) [NBP3-13233]
Western Blot: Caspase-1 Antibody - (cleaved) [NBP3-13233] - Untreated (-) and treated (+) THP-1 whole cell extracts and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with Caspase-1 (cleaved Asp297) antibody (NBP3-13233) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Western Blot: Caspase-1 Antibody(cleaved) [NBP3-13233]
Western Blot: Caspase-1 Antibody - (cleaved) [NBP3-13233] - Treated (+) THP-1 whole cell extracts and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cleaved Caspase-1 (p20) antibody (NBP3-13233) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for Caspase-1 Antibody - (cleaved)
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-1
Given the role of IL-1beta in inflammation, it makes sense that many diseases and pathologies have been associated with dysregulation of caspase-1 activation and the inflammasome (3, 4). The inflammasome is a multiprotein complex comprised of Nod-like receptor (NLR) family members and the adapter ASC (apoptosis-associated speck-like protein containing a CARD) which are crucial for capase-1 activation (3-5). For instance, the neuronal apoptosis inhibitory protein (NAIP)/NLRC4 inflammasome has been associated with colorectal cancer, breast cancer, and glioma pathogenesis (5). Caspase-1 activation and mutations in the inflammasome have also been linked to Chron's disease and Alzheimer's disease (4). In addition to immune and inflammatory related disorder, the inflammasome has been linked to metabolic and obesity related disorders including diabetes and cardiovascular disease (6). Finally, caspase-1 deficient mice exhibit enhanced epithelial cell proliferation in the colon, increased tumor formation, and reduced apoptosis (1). A more thorough understanding of the inflammasome-caspase-1 signaling pathway will be important for understanding disease pathology and potential therapeutic development.
Alternative names for caspase-1 includes CASP1, CASP1 nirs variant 1, EC 3.4.22.36, ICE, IL-1 beta-converting enzyme, IL1BC, IL1BCE, IL1B-converstase, interleukin-1 beta convertase, and p45.
References
1. Shalini, S., Dorstyn, L., Dawar, S., & Kumar, S. (2015). Old, new and emerging functions of caspases. Cell death and differentiation. https://doi.org/10.1038/cdd.2014.216
2. Chang, H. Y., & Yang, X. (2000). Proteases for cell suicide: functions and regulation of caspases. Microbiology and molecular biology reviews: MMBR. https://doi.org/10.1128/mmbr.64.4.821-846.2000
3. Vanaja, S. K., Rathinam, V. A., & Fitzgerald, K. A. (2015). Mechanisms of inflammasome activation: recent advances and novel insights. Trends in cell biology. https://doi.org/10.1016/j.tcb.2014.12.009
4. Franchi, L., Eigenbrod, T., Munoz-Planillo, R., & Nunez, G. (2009). The inflammasome: a caspase-1-activation platform that regulates immune responses and disease pathogenesis. Nature immunology. https://doi.org/10.1038/ni.1703
5. Kay, C., Wang, R., Kirkby, M., & Man, S. M. (2020). Molecular mechanisms activating the NAIP-NLRC4 inflammasome: Implications in infectious disease, autoinflammation, and cancer. Immunological reviews. https://doi.org/10.1111/imr.12906
6. Pham, D., Park, P. (2020). Recent insights on modulation of inflammasomes by adipokines: a critical event for the pathogenesis of obesity and metabolism-associated diseases. Archives of Pharmacal Research. https://doi.org/10.1007/s12272-020-01274-7
Alternate Names
CASP1, Caspase1, ICE
Gene Symbol
CASP1
Additional Caspase-1 Products
Product Documents for Caspase-1 Antibody - (cleaved)
Product Specific Notices for Caspase-1 Antibody - (cleaved)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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