Caspase-4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13397
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caspase-4. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Caspase-4 Antibody
Western Blot: Caspase-4 Antibody [NBP3-13397]
Western Blot: Caspase-4 Antibody [NBP3-13397] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase-4 antibody (NBP3-13397) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: Caspase-4 Antibody [NBP3-13397]
Immunohistochemistry-Paraffin: Caspase-4 Antibody [NBP3-13397] - Rat kidney. Caspase-4 stained by Caspase-4 antibody (NBP3-13397) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Western Blot: Caspase-4 Antibody [NBP3-13397]
Western Blot: Caspase-4 Antibody [NBP3-13397] - THP-1 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase-4 (cleaved Gln81) antibody (NBP3-13397) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for Caspase-4 Antibody
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-4
Alternate Names
CASP4, Caspase4, ICE(rel)II, ICH-2, Mih1/TX, TX
Gene Symbol
CASP4
Additional Caspase-4 Products
Product Specific Notices for Caspase-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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