Caspase 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87682

Novus Biologicals, part of Bio-Techne
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NBP1-87682
NBP1-87682-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Caspase-5 Antibodies »

Product Summary for Caspase 5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids 8 - 86 of human Caspase-5: VEGVFIFLIEDSGKKKRRKNFEAMFKGILQSGLDNFVINHMLKNNVAGQTSIQTLVPNTDQKSTSVKKDNHKKKTVKMLEYLGKDV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase 5 Antibody

Immunohistochemistry-Paraffin: Caspase 5 Antibody [NBP1-87682]

Immunohistochemistry-Paraffin: Caspase 5 Antibody [NBP1-87682]

Immunohistochemistry-Paraffin: Caspase 5 Antibody [NBP1-87682] - Staining of human liver shows strong nuclear positivity in hepatocytes.

Applications for Caspase 5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-5

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-5 (ICH-3, ICEREL-III, TY protease) is a member of the caspase-1 subfamily and it is an upstream mediator of apoptosis. Caspase-5 is highly expressed in lung, liver, and skeletal muscle, and similar to Caspase-4, its overexpression can induce cellular apoptosis (1). A tetramer, Caspase-5 separates into two subunits of a 20kda (p20) and 10kda (p10) after activation. In vitro, Caspase-5 can be cleaved by GranB, a major player in Cytotoxic T lymphocytes (CTL) induced apoptosis (2). In vivo, the Caspase-5 expression is elevated by LPS and IFNg. Caspase-5 induced apoptosis can be inhibited by CrmA and Bcl-2. Caspase-5 has also been implicated in cellular inflammatory response and in the microsatelite mutator pathway for cancer (3).

Alternate Names

CASP5, Caspase5, ICE(rel)III, Protease ICH-3, Protease TY

Entrez Gene IDs

838 (Human)

Gene Symbol

CASP5

UniProt

P51878 (Human)

Additional Caspase-5 Products

Product Documents for Caspase 5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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