Caspase-7 Antibody (25B881.1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56529
![Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]](https://resources.bio-techne.com/images/products/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0008.jpg "Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 25B881.1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was generated against recombinant protein of active form of human Caspase-7.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Caspase-7 Antibody (25B881.1) - BSA Free
Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]
Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.![Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]](https://resources.bio-techne.com/images/products/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0009.jpg "Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]")
Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]
Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.![Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) - BSA Free [NB100-56529]](https://resources.bio-techne.com/images/products/Caspase-7-Antibody-25B881-1-Immunohistochemistry-Paraffin-NB100-56529-img0007.jpg "Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) - BSA Free [NB100-56529]")
Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) - BSA Free [NB100-56529]
Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) [NB100-56529] - Human tonsil stained with Caspase-7 antibody at 5 ug/ml. Novus' human tonsil tissue slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Applications for Caspase-7 Antibody (25B881.1) - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
In Jurkat, a 35 kDa band is observed.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-7
Additional Caspase-7 Products
Product Documents for Caspase-7 Antibody (25B881.1) - BSA Free
Product Specific Notices for Caspase-7 Antibody (25B881.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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