Caspase-9 Antibody (9M6N2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15590

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15590-100ul
NBP3-15590-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9M6N2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Caspase-9 Antibodies »

Product Summary for Caspase-9 Antibody (9M6N2)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 139-416 of Caspase-9 (NP_001220.2). VGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-9 Antibody (9M6N2)

Western Blot: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Western Blot: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Caspase-9 Rabbit mAb (NBP3-15590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Western Blot: Caspase-9 Antibody (9M6N2) [NBP3-15590]

Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.
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Applications for Caspase-9 Antibody (9M6N2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-9

Caspase 9 (CASP9) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. Caspase 9 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 9 form.

There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Caspase 3 is considered an initiator, as initiated caspase-9 goes on to cleave the effector procaspase-3 and procaspase-7.

Caspase 9 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Alternate Names

CASP9, Caspase9

Gene Symbol

CASP9

Additional Caspase-9 Products

Product Documents for Caspase-9 Antibody (9M6N2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase-9 Antibody (9M6N2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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