Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LAP6 96-2-22

Format

BSA Free

Concentration

1.0 mg/ml

View all Caspase-9 Antibodies »

Product Specifications

Immunogen

Recombinant human Caspase 9 pro-domain protein. [UniProt# P55211]

Localization

Cytoplasmic and Mitochondrial

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Caspase-9 Antibody (LAP6 96-2-22) - BSA Free

Western Blot: Caspase-9 Antibody (LAP6 96-2-22)BSA Free [NB500-209]

Western Blot: Caspase-9 Antibody (LAP6 96-2-22)BSA Free [NB500-209]

Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - Western blot analysis of Jurkat (A) and HeLa (B) cell lysate using Caspase 9 (NB500-209) antibody at 1:500.
Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody (LAP6 96-2-22) - BSA Free [NB500-209]

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody (LAP6 96-2-22) - BSA Free [NB500-209]

Immunocytochemistry/Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - The Caspase 9 antibody was tested in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).
Immunohistochemistry-Paraffin: Caspase-9 Antibody (LAP6 96-2-22) - BSA Free [NB500-209]

Immunohistochemistry-Paraffin: Caspase-9 Antibody (LAP6 96-2-22) - BSA Free [NB500-209]

Immunohistochemistry-Paraffin: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - IHC analysis of paraffin-embedded human breast carcinomausing Caspase 9 antibody at 1:25. Image from verified customer review.
View 2 more images

Applications for Caspase-9 Antibody (LAP6 96-2-22) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

8-25 ug/ml. Use reported by customer review

Immunohistochemistry-Paraffin

8-25 ug/ml

Simple Western

20 ug/ml

Western Blot

1 ug/ml
Application Notes
In Western Blot, bands at ~46kDa-48kDa and 35kDa are seen representing the pro and active forms of Caspase 9, respectively. In ICC/IF cytoplasmic staining can be seen in HeLa cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.8 using NB500-209 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Caspase-9

Among the initiator caspases (caspases -2, -8 and -9), caspase-9 is an essential enzymes required for intrinsic or mitochondrial apoptotic pathway. Caspase 9 is a heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kD (p35) and a 10 kD (p10) subunit, and it interacts with several proteins including APAF1, EFHD2, HAX1, BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce, BIRC7/livin etc. During apoptosome-dependent apoptotic execution, cytochrome-c is released from the mitochondria into cytosol where it binds/activates Apaf-1. Upon nucleotide exchange in the presence of dATP, Apaf-1 oligomerizes into heptameric apoptosome complex and procaspase-9 is recruited to apoptosome where it gets activated. Active caspase-9 at the apoptosome proteolytically activates procaspase-3 (the critical apoptotic executioner caspases) and cleaves poly(ADP-ribose) polymerase (PARP). Both caspases-9 and -3 can be inhibited by selected members of IAPs, with XIAP being their most potent natural inhibitor. Phosphorylation of a major site, Thr125 by CDK1-cyclin B1 leading to inhibition of caspase 9 plays a critical role in the restraint of apoptosis during mitotic arrest. This site is also targeted by ERK1/2 in response to growth factor signalling and by DYRK1A, which plays important roles during development.

Alternate Names

CASP9, Caspase9

Entrez Gene IDs

842 (Human)

Gene Symbol

CASP9

UniProt

P55211

Additional Caspase-9 Products

Product Documents for Caspase-9 Antibody (LAP6 96-2-22) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase-9 Antibody (LAP6 96-2-22) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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