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Caspase-9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56118

Novus Biologicals, part of Bio-Techne
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NB100-56118
NB100-56118SS

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Gerbil

Cited:

Human, Mouse, Rat, Canine

Applications

Validated:

Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry Control, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Human Caspase 9 recombinant protein catalytic subunits (NP_001220).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase-9 Antibody

Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Mice with two different spontaneous mutant alleles of Sharpin show variations in onset of CPDM phenotype. However, the onset is much more rapid in Sharpincpdm-Dem mice as evidenced by antibody detection of cleaved CASPASE 9 (T) when compared to Sharpincpdm mutants (R) or WT mice (Q,S). . Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085666) licensed under a CC-BY license.
Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Increased the expression of Caspase-9 in 12-week-old Atg7deltapan mice. Caspase-9 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (green) (1/1000, scale bar=50?um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Capase-9 in mouse transplanted lung tumor section using anti-Caspase-9 antibody. Image from verified customer review.

Applications for Caspase-9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 22223358)

Immunohistochemistry-Frozen

Assay Dependent

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

reported in scientific literature (PMID 24184144)
Application Notes
Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active/cleaved Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation: the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.

Reviewed Applications

Read 1 review rated 5 using NB100-56118 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-9

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNF-alpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Alternate Names

CASP9, Caspase9

Entrez Gene IDs

842 (Human); 58918 (Rat)

Gene Symbol

CASP9

UniProt

Additional Caspase-9 Products

Product Documents for Caspase-9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Caspase-9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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