Caspase-9 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56118
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Gerbil
Cited:
Human, Mouse, Rat, Canine
Applications
Validated:
Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry Control, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Human Caspase 9 recombinant protein catalytic subunits (NP_001220).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Caspase-9 Antibody
Immunohistochemistry: Caspase-9 Antibody [NB100-56118]
Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Mice with two different spontaneous mutant alleles of Sharpin show variations in onset of CPDM phenotype. However, the onset is much more rapid in Sharpincpdm-Dem mice as evidenced by antibody detection of cleaved CASPASE 9 (T) when compared to Sharpincpdm mutants (R) or WT mice (Q,S). . Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085666) licensed under a CC-BY license.Immunohistochemistry: Caspase-9 Antibody [NB100-56118]
Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Increased the expression of Caspase-9 in 12-week-old Atg7deltapan mice. Caspase-9 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (green) (1/1000, scale bar=50?um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Capase-9 in mouse transplanted lung tumor section using anti-Caspase-9 antibody. Image from verified customer review.Applications for Caspase-9 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 22223358)
Immunohistochemistry-Frozen
Assay Dependent
Immunohistochemistry-Paraffin
1:1000-1:5000
Immunoprecipitation
1:50-1:200
Western Blot
reported in scientific literature (PMID 24184144)
Application Notes
Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active/cleaved Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation: the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.
Reviewed Applications
Read 1 review rated 5 using NB100-56118 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-9
Alternate Names
CASP9, Caspase9
Gene Symbol
CASP9
UniProt
Additional Caspase-9 Products
Product Documents for Caspase-9 Antibody
Product Specific Notices for Caspase-9 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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