Caspase-9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56119

Novus Biologicals, part of Bio-Techne
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NB100-56119
NB100-56119SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Mouse, Rat, Canine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Caspase-9 Antibodies »

Product Specifications

Immunogen

Recombinant mutated non-cleavable pro form of human Caspase 9 (NP_001220).

Specificity

This polyclonal antisera recognizes the proform of caspase-9 (~50 kDa), and the large (~35 kDa) and small (~15 kDa) subunits of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-9 Antibody

Western Blot: Caspase-9 Antibody [NB100-56119]

Western Blot: Caspase-9 Antibody [NB100-56119]

Western Blot: Caspase-9 Antibody [NB100-56119] - Analysis of Caspase-9. Various tissue lysates were prepared from human autospsy material and normalized for total protein. Most tissues contained the ~50 kDa pro-Caspase-9 protein. Cleaved Caspase-9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9: recombinant human pro-Caspase-9 (full-length) protein. PRO: pro-Caspase-9. L: large subunit of cleaved Caspase-9. S: small subunit of cleaved Caspase-9.
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56119]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56119]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56119] - Mouse brain tissue sections at 1:2000. Hematoxylin-eosin counterstain. A) Brain striatum, B) Brain stem motor neuron.
Simple Western: Caspase-9 Antibody [NB100-56119]

Simple Western: Caspase-9 Antibody [NB100-56119]

Simple Western: Caspase-9 Antibody [NB100-56119] - Lane view shows a specific band for Caspase 9 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Applications for Caspase-9 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Simple Western

1:200

Western Blot

1:1000-1:2000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

50 ul neat whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-9

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-9 polyclonal antisera recognizes the proform of caspase-9 (approx. 50 kDa), and the large (approx. 35 kDa) and small (approx. 15 kDa) subunits of active/cleaved Caspase-9.

Alternate Names

CASP9, Caspase9

Entrez Gene IDs

842 (Human); 12371 (Mouse); 58918 (Rat)

Gene Symbol

CASP9

UniProt

P55211

Additional Caspase-9 Products

Product Documents for Caspase-9 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase-9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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