Cathepsin B Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19797

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Cathepsin B Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the Cathepsin B human protein (between residues 200-270) [UniProt P07858]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: bovine (84%) and chicken (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cathepsin B Antibody - BSA Free

Western Blot: Cathepsin B AntibodyBSA Free [NBP1-19797]

Western Blot: Cathepsin B AntibodyBSA Free [NBP1-19797]

Western Blot: Cathepsin B Antibody [NBP1-19797] - extracts from COLO cells.
Immunocytochemistry/ Immunofluorescence: Cathepsin B Antibody - BSA Free [NBP1-19797]

Immunocytochemistry/ Immunofluorescence: Cathepsin B Antibody - BSA Free [NBP1-19797]

Immunocytochemistry/Immunofluorescence: Cathepsin B Antibody [NBP1-19797] - ICC/IF analysis of HeLa cells using 1:50 dilution of Cathepsin B antibody followed by detection using Dylight 488 labelled secondary antibody (green). Nuclei and alpha-tubulin were counterstained using DAPI (blue) and Dylight 550 (red) respectively. Punctate staining was observed for Cathepsin B indicating its presence in the cellular lysosomes, endosomes and melanosomes. Image objective 40x.
Immunohistochemistry-Paraffin: Cathepsin B Antibody - BSA Free [NBP1-19797]

Immunohistochemistry-Paraffin: Cathepsin B Antibody - BSA Free [NBP1-19797]

Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-19797] - Human brain tissue.
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Applications for Cathepsin B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1.0 - 2.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin B

The cathepsin protease family contains several diverse classes of enzymes. Cathepsins B, L, H, K, S and O are part of the cysteine protease class. Cathepsin B is a dimer consisting of a heavy chain and a light chain cross-linked by a disulfide bond and is a lysosomal cysteine protease. Cathepsin B is expressed in luminal epithelial cells and its expression indicates that Cathepsin B is a marker for secretory cell death. Cathepsin B also participates in intracellular degradation and turnover of proteins.

Alternate Names

CTSB

Entrez Gene IDs

1508 (Human); 13030 (Mouse); 64529 (Rat)

Gene Symbol

CTSB

UniProt

P07858

Additional Cathepsin B Products

Product Documents for Cathepsin B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cathepsin B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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