Cathepsin F Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1784

Novus Biologicals, part of Bio-Techne
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NB100-1784
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Cathepsin F Antibodies »

Product Specifications

Immunogen

Peptide with sequence CGVNTMASSAVVD corresponding to C-Terminus according to NP_003784.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cathepsin F Antibody

Western Blot: Cathepsin F Antibody [NB100-1784]

Western Blot: Cathepsin F Antibody [NB100-1784]

Western Blot: Cathepsin F Antibody [NB100-1784] - (0.3ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Cathepsin F Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Experiments gave bands at approx. 30 kDa band and 35 kDa band in human prostate and ovary lysates after 0.3ug/ml antibody staining. These bands correspond to earlier findings are reported in scientific literature with different antibodies (PMID: 9822672). This protein has a calculated MW of 53.4 kDa band according to NP_003784.2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cathepsin F

Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W.

Alternate Names

CTSF

Entrez Gene IDs

8722 (Human)

Gene Symbol

CTSF

UniProt

NP_003784.2 (Human)

Additional Cathepsin F Products

Product Documents for Cathepsin F Antibody

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Certificate of Analysis

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Product Specific Notices for Cathepsin F Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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