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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # S48

Concentration

1 mg/ml

Product Specifications

Immunogen

Fusion protein amino acids 859-875 of rat Cav1.3

Localization

Membrane, Cell Membrane

Specificity

Detects approx 250 kDa. No cross-reactivity against Cav1.2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Cav1.3 Antibody (S48)

Immunocytochemistry/ Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893]

Immunocytochemistry/ Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893]

Immunocytochemistry/Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3Monoclonal Antibody (NBP2-12893) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3Antibody (D) Composite.
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]

Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]

Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody (NBP2-12893) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]

Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]

Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - IHC analysis of Cav1.3 tested on human hippocampus lysates. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

Applications for Cav1.3 Antibody (S48)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml\L of Cav1.3 Antibody was sufficient for detection of Cav1.3 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cav1.3

1 ug/mL was sufficient for detection of Cav1.3 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Long Name

Voltage-dependent L-type calcium channel subunit alpha-1D

Alternate Names

CACH3, CACN4, CACNA1D, CACNL1A2, CCHL1A2

Gene Symbol

CACNA1D

Additional Cav1.3 Products

Product Documents for Cav1.3 Antibody (S48)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cav1.3 Antibody (S48)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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