Caveolin-1 Antibody (2A7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26695
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2A7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Caveolin-1 [UniProt Q03135]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Caveolin-1 Antibody (2A7)
Western Blot: Caveolin-1 Antibody (2A7) [NBP3-26695] -
Western Blot: Caveolin-1 Antibody (2A7) [NBP3-26695] - Positive Western Blot detected in: THP-1 whole cell lysate.All lanes: Caveolin-1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Flow Cytometry: Caveolin-1 Antibody (2A7) [NBP3-26695] -
Flow Cytometry: Caveolin-1 Antibody (2A7) [NBP3-26695] - Overlay Peak curve showing Hela cells stained with Caveolin-1 Antibody (2A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for Caveolin-1 Antibody (2A7)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Caveolin-1
Alternate Names
CAV1, Caveolin1, MSTP085, VIP21
Gene Symbol
CAV1
Additional Caveolin-1 Products
Product Documents for Caveolin-1 Antibody (2A7)
Product Specific Notices for Caveolin-1 Antibody (2A7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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