CBL Antibody (3B12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37574

Novus Biologicals, part of Bio-Techne
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NBP2-37574
NBP2-37574-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3B12

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CBL Antibodies »

Product Summary for CBL Antibody (3B12) - BSA Free

Immunogen

Purified recombinant fragment of human CBL expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CBL Antibody (3B12) - BSA Free

Western Blot: CBL Antibody (3B12)BSA Free [NBP2-37574]

Western Blot: CBL Antibody (3B12)BSA Free [NBP2-37574]

Western Blot: CBL Antibody (3B12) [NBP2-37574] - Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunocytochemistry/ Immunofluorescence: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunocytochemistry/Immunofluorescence: CBL Antibody (3B12) [NBP2-37574] - Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunohistochemistry-Paraffin: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunohistochemistry-Paraffin: CBL Antibody (3B12) [NBP2-37574] - Analysis of cancer tissues using C-CBL mouse mAb with DAB staining.
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Applications for CBL Antibody (3B12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-37574 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CBL

c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).

Long Name

Casitas B-lineage Lymphoma, E3 Ubiquitin Protein Ligase CBL

Alternate Names

CBL2, RNF55

Gene Symbol

CBL

Additional CBL Products

Product Documents for CBL Antibody (3B12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CBL Antibody (3B12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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