Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Cbl-B Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 450 and 500 of human Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence b using the numbering given in entry NP_733762.2 (GeneID 868).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CBLB Antibody

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919]

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919]

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Cbl-b used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: CBLB Antibody [NBP1-49919]

Immunoprecipitation: CBLB Antibody [NBP1-49919]

Immunoprecipitation: CBLB Antibody [NBP1-49919] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody NBP1-49919 used for IP at 3 ug/mg lysate. Cbl-b was also immunoprecipitated by rabbit anti-Cbl-b antibody NBP1-49920, which recognizes a downstream epitope. For blotting immunoprecipitated Cbl-b, NBP1-49920 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for CBLB Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cbl-B

CBLB is an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.

Long Name

Cbl Proto-Oncogene B

Alternate Names

CBLB, Nbla00127, RNF56

Gene Symbol

CBLB

Additional Cbl-B Products

Product Documents for CBLB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CBLB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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