CBR1 Antibody (4E12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74041

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4E12

Format

BSA Free

Concentration

1 mg/ml

View all CBR1 Antibodies »

Product Summary for CBR1 Antibody (4E12) - BSA Free

Immunogen

Recombinant human CBR1 (1-277 aa ) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CBR1 Antibody (4E12) - BSA Free

Western Blot: CBR1 Antibody (4E12)BSA Free [NBP1-74041]

Western Blot: CBR1 Antibody (4E12)BSA Free [NBP1-74041]

Western Blot: CBR1 Antibody (4E12) [NBP1-74041] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR1 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: CBR1 Antibody (4E12) - BSA Free [NBP1-74041]

Immunocytochemistry/ Immunofluorescence: CBR1 Antibody (4E12) - BSA Free [NBP1-74041]

Immunocytochemistry/Immunofluorescence: CBR1 Antibody (4E12) [NBP1-74041] - Analysis of CBR1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-CBR1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for CBR1 Antibody (4E12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CBR1

Carbonyl reductase 1 (CBR1) is a NADPH-dependent, monomeric, and cytosolic enzyme belonging to a family of short-chain dehydrogenases/reductases. This protein consists of 277 amino acid residues and is widely distributed in human tissues such as liver, epidermis, stomach, small intestine, kidney, neuronal cells, and smooth muscle fiber. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer drug, doxorubicin. The best substrates of CBR1 are quinones, including ubiquinone-1 and tocophrolquinone (vitamin E).

Alternate Names

15-hydroxyprostaglandin dehydrogenase [NADP+], carbonyl reductase (NADPH) 1, carbonyl reductase (NADPH) 1, EC 1.1.1.18410EC 1.1.1.197,15-hydroxyprostaglandin dehydrogenase, carbonyl reductase [NADPH] 1, carbonyl reductase 1, CBR, CRN, EC 1.1.1.184, EC 1.1.1.189, hCBR1, NADPH-dependent carbonyl reductase 1, Prostaglandin 9-ketoreductase, Prostaglandin-E(2) 9-reductase, SDR21C1, short chain dehydrogenase/reductase family 21C, member 1

Gene Symbol

CBR1

UniProt

NP_001748 (Human)

Additional CBR1 Products

Product Documents for CBR1 Antibody (4E12) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CBR1 Antibody (4E12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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