We would like to check on Cystathionine beta synthase # H00000875-M01. We need a mouse monoclonal. Applications: Immunoblotting, IHC May I know if this can cross-react with mouse CBS? Because we are actually probing this enzyme in a mouse cell line / tissue and the species is human, host is mouse.

This antibody has never been tested against mouse samples so we don't know if it will cross react. I recommend checking with a blast against the mouse protein to find out how similar the immunogen is to the mouse protein. If it is 80% homologous or higher typically there is a good chance it might react. In addition to using an anti-mouse secondary will bind non-specifically to their mouse tissues, and most likely cause high background. Running a secondary only control would be advised or even better increase the concentration of the primary on their samples and use a directly conjugated version to avoid non-specific secondary binding. In this case we can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If testing on this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product of equal value in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.

I was wondering if the CBS Antibody (3E1) (H00000875-M01) has been used for immunofluorescence on monolayer cell cultures?

Unfortunately it has never been tested for immunofluorescence in an ICC application on cells. Since it stains tissue, I would imagine it should be capable of staining cells as well. Without testing we cannot guarantee this, but if you want to test using our Innovators Reward Program to save some money for providing feedback that is an option for you. Here are the details: If you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. The great thing is you are eligible regardless if you get positive results or not.

CBS Antibody (3E1)

Name: CBS Antibody (3E1)
Brand: Novus Biologicals
SKU: H00000875-M01
Rating: 3 (1 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # H00000875-M01

(1)

Citations (53)

Product Datasheet / COA / SDS

Discontinued Product

H00000875-M01 has been discontinued. View all CBS products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bacteria

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CBS Antibodies »

Product Specifications

Immunogen

CBS (NP_000062, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEPLWIRPDAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFG

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24611772). Mouse reactivity reported in scientific literature (PMID: 22419888).

Specificity

CBS - cystathionine-beta-synthase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CBS Antibody (3E1)

Western Blot: CBS Antibody (3E1) [H00000875-M01]

Western Blot: CBS Antibody (3E1) [H00000875-M01] - Analysis of CBS expression in transfected 293T cell line by CBS monoclonal antibody (M01), clone 3E1.Lane 1: CBS transfected lysate(61 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: CBS Antibody (3E1) [H00000875-M01]

Immunohistochemistry-Paraffin: CBS Antibody (3E1) [H00000875-M01] - Analysis of monoclonal antibody to CBS on formalin-fixed paraffin-embedded human hepatocellular carcinoma. Antibody concentration 3 ug/ml.

Western Blot: CBS Antibody (3E1) [H00000875-M01]

Western Blot: CBS Antibody (3E1) [H00000875-M01] - Analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi ( Cat # H00000875-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CBS monoclonal antibody (M01), clone 3E1 (Cat # H00000875-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

View 4 more images

Applications for CBS Antibody (3E1)

Application

Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500

Application Notes

Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA. ICC/IF usage reported in scientific literature (PMID: 21571312).

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using H00000875-M01 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CBS

The protein encoded by this gene is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.

Alternate Names

cystathionine-beta-synthase, EC 4.2.1.2210Beta-thionase

Entrez Gene IDs

875 (Human)

Gene Symbol

CBS

OMIM

236200 (Human)

UniProt

NP_000062 (Human)

Additional CBS Products

Product Documents for CBS Antibody (3E1)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CBS Antibody (3E1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

CBS Antibody (3E1)

Novus Biologicals, part of Bio-Techne | Catalog # H00000875-M01

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all CBS Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Specificity

Clonality

Host

Isotype

Description

Scientific Data Images for CBS Antibody (3E1)

Western Blot: CBS Antibody (3E1) [H00000875-M01]

Immunohistochemistry-Paraffin: CBS Antibody (3E1) [H00000875-M01]

Western Blot: CBS Antibody (3E1) [H00000875-M01]

Western Blot: CBS Antibody (3E1) [H00000875-M01]

Western Blot: CBS Antibody (3E1) [H00000875-M01]

Immunoprecipitation: CBS Antibody (3E1) [H00000875-M01]

ELISA: CBS Antibody (3E1) [H00000875-M01]

Applications for CBS Antibody (3E1)

Immunohistochemistry

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CBS

Alternate Names

Entrez Gene IDs

Gene Symbol

OMIM

UniProt

Additional CBS Products

Product Documents for CBS Antibody (3E1)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CBS Antibody (3E1)