Ccd1/DIXDC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12691

Novus Biologicals, part of Bio-Techne
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NBP3-12691-100ul
NBP3-12691-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ccd1/DIXDC1 Antibodies »

Product Summary for Ccd1/DIXDC1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human Ccd1/DIXDC1 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for Ccd1/DIXDC1 Antibody

Western Blot: Ccd1/DIXDC1 Antibody [NBP3-12691]

Western Blot: Ccd1/DIXDC1 Antibody [NBP3-12691]

Western Blot: Ccd1/DIXDC1 Antibody [NBP3-12691] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:77 kDa
Immunocytochemistry/ Immunofluorescence: Ccd1/DIXDC1 Antibody [NBP3-12691]

Immunocytochemistry/ Immunofluorescence: Ccd1/DIXDC1 Antibody [NBP3-12691]

Immunocytochemistry/Immunofluorescence: Ccd1/DIXDC1 Antibody [NBP3-12691] - Staining of DIXDC1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human DIXDC1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunoprecipitation: Ccd1/DIXDC1 Antibody [NBP3-12691]

Immunoprecipitation: Ccd1/DIXDC1 Antibody [NBP3-12691]

Immunoprecipitation: Ccd1/DIXDC1 Antibody [NBP3-12691] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-DIXDC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-DIXDC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 77 kDaObserved band size :95 kDa

Applications for Ccd1/DIXDC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ccd1/DIXDC1

DIXDC1 (DIX domain containing 1/Dixin) is a 683 amino acid protein. It positively regulates the Wnt signaling pathway by activating WNT3A via DVL2. It is expressed ubiquitously but has higher expression in skeletal and cardiac muscle. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation. DIXDC1 may play a role in breast cancer, breast carcinoma, endometrial cancer, epithelial neoplasia, schizophrenia, colon cancer, and neuroblastoma.

Long Name

Coiled-coil DIX1/DIX Domain Containing 1

Alternate Names

Dixdc1, Dixin, Kiaa1735

Gene Symbol

DIXDC1

Additional Ccd1/DIXDC1 Products

Product Documents for Ccd1/DIXDC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ccd1/DIXDC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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